An Apparatus for Preparative Electrophoresis
نویسندگان
چکیده
منابع مشابه
A new apparatus for preparative gel electrophoresis.
paratus is the dimension of the elution cham ber; if a large chamber is employed, dilution of solute occurs thus resulting in broadening of the elution patterns, while with a narrow chamber elution is frequently interrupted by the swelling of gel during operation. In many cases, either upward or downward elution has been employed and this leads to com plication of the apparatus. This communi ca...
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Electrophoresis is widely used as a separative technique, particularly in protein chemistry. In some respects gelatinous media have an advantage over others as support media for electrophoresis, perhaps most obviously when a gel matrix of molecular dimensions is employed, allowing the simultaneous use of two properties for separation of the particles of interest-size and surface charge density....
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A method of preparative electrophoresis is described. A volume of approximately 1 ml. of protein solution can be applied with the cell used. A “marker” solution, applied adjacent to the sample to be separated, indicates the positions of the protein bands during the migration. The procedure has the advantages of a high degree of recovery, simplicity of operation, and utilization of an electropho...
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‘T1HE INTRODUC”ION of starch gel as an electrophoresis medimn by Smithies k4) arkecl a significant advance in the analysis of serum proteins. The increased resolving power afforded by this medium is indicated by the fact that as many as 30 distinct components can be electrophoretically separated; only 5 or 6 are usually observed on paper or in free-solution electrophoresis. Acrylamide gel (2, 3...
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ژورنال
عنوان ژورنال: Nature
سال: 1947
ISSN: 0028-0836,1476-4687
DOI: 10.1038/160469a0